. Author manuscript; available in PMC: 2012 Dec 23.

Published in final edited form as: Pediatr Res. 2012 Sep 24;72(6):568–575. doi: 10.1038/pr.2012.124

Table 1.

Primary antibodies and lectins used in this study

Antibody	Clone	Catalog number	Dilution
Rabbit monoclonal antiphospho-ERK1/2 (Thr202/Tyr204)	D13.14.4E	CST 4370	1:2,000 (WB); 1:200 (IF)
Rabbit monoclonal antiphospho-Akt Ser473	193H12	CST 4058	1:1,000 (WB)
Rabbit polyclonal anti-ERK1/2		CST 9102	1:1,000 (WB)
Rabbit polyclonal anti-Akt		CST 9272	1:1,000 (WB)
Rabbit polyclonal antimouse HIF-1α		NB100-479	1:1,000 (WB)
Mouse monoclonal anti-HIF-1β	H1β234	NB100-124	1:500 (WB)
Mouse monoclonal anti-β-actin	AC-74	A5316	1:20,000 (WB)
Mouse monoclonal antineuronal nuclei	A60	MAB377	1:200 (IF)
Mouse monoclonal antiglial fibrillary acidic protein	GA5	MAB360	1:500 (IF)
Mouse monoclonal anti-2′,3′-cyclic nucleotide 3′-phosphodiesterase	11-5B	MAB326	1:200 (IF)
Isolectin IB4, Alexa Fluor 488 conjugate			1:500 (IF)

A, Sigma; CST, Cell Signaling Technology; ERK, extracellular-regulated kinase; HIF, hypoxia-inducible factor; IB4, isolectin B4; IF, immunofluorescence; MAB, Chemicon; NB, Novus Biological; WB, western blotting.