Fig. 2.

Immunomodulatory effects of interferon-γ (IFN-γ) on allergen-specific interleukin-10 (IL-10)-producing regulatory B cell (Br1) responses in peripheral blood mononuclear cells (PBMCs). (A) Representative plot of allergen-specific Br1 responses of cells from milk allergy patients and milk-tolerant subjects induced by stimulation with casein and/or IFN-γ. Numbers indicate the percentage of cells. (B) Allergen-specific changes in Br1 cell numbers in milk allergy patients and milk-tolerant subjects. (C) Changes in the proportion of Br1 cells to CD5(+) B cells as a result of IFN-γ treatment. Positive, milk allergy patient (DBPCFC positive); Negative, milk-tolerant subject (DBPCFC negative); None, unstimulated PBMCs; Casein, PBMCs stimulated with casein; IFN+Casein, PBMCs stimulated with IFN-γ and casein simultaneously.