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. 2012 Nov 1;6(1):184–196. doi: 10.1242/dmm.009563

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© 2012. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 3. — Expression of expanded CTG repeats modifies TBPH localization in the Drosophila muscle. (A–C) Fluorescent confocal images of rostrocaudal cryosections from adult Drosophila thoraces stained with an anti-TBPH antibody (green), and counterstained with phalloidin (red). In control flies (Mhc>yw; A) TBPH was detected preferentially in the cytoplasm, as a part of the sarcomeric H-bands. (B) Expression of i(CTG)480 in the muscle [Mhc-Gal4 UAS-i(CTG)480] enhanced the TBPH signal in the sarcomeric Z-bands. (C) Coexpression of i(CTG)480 with human MBNL1 [Mhc-Gal4 UAS-i(CTG)480>UAS-MBNL1] partially rescued the sarcomeric localization of TBPH. (D) Representation of the basic organization of a sarcomere. (E–L) Fluorescent confocal images comparing the subcellular distribution of i(CUG)480 RNA (fluorescent in situ hybridization using a CAG red-labeled probe; E,I) with TBPH protein (green; G,K; see merge in H,L), in control (E–H) and CTG-expressing flies (I–L). Expression of CTG repeats in the muscle caused a marked increase in nuclear TBPH, which did not seem to colocalize with nuclear CUG-RNA foci. Nuclei were counterstained with DAPI (blue; F,J).