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. 2012 Nov 26;154(1):25–35. doi: 10.1210/en.2012-1728

Fig. 4.

Fig. 4.

Demethylating agents 5′-aza-CdR and zebularine decrease cell proliferation by attenuating β-catenin signaling in FTC-236. A, Western blot analysis of p-β-catenin (serine 552), cyclin D1, and GAPDH as the loading control in whole-cell lysates from FTC-236 cells after treatment with demethylating agents. B, Quantification of relative protein abundance of p-β-catenin (serine 552) and cyclin D1 after normalization by using GAPDH as a loading control. C, Western blot analysis of protein abundance of β-catenin in the nuclear and cytoplasm cell fractions. PARP was used as loading control for nuclear fraction and α-tubulin was used as loading control for cytoplasmic fractions of FTC-236 cells. D, Western blot analysis of p-β-catenin (serine 552), total β-catenin, cyclin D1, and GAPDH as the loading control in human tissue lysates of normal thyroid and cancer samples.