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A-III metabolism by HAC15 cells. Confluent cells were serum deprived in DMEM:F12 containing 0.1% Cosmic Calf serum for 24 h. After three times washing by HEPES buffer, HEPES buffer with A-III (100 nm) was incubated for indicated time. Buffer was removed, extracted, and analyzed for A-II peptides by LC-MS. The study was done twice. **, P < 0.01; *, P < 0.05.
