Fig. 7.

Association of GR and ERα with the promoter of GILZ. A, Recruitment of GR and ERα to GRE at position −1919 to −1794 was assessed by ChIP assay after treatment with vehicle (Veh), 100 nm Dex, 10 nm E₂, or 100 nm Dex and 10 nm E₂ (D + E₂) for 1 h in ECC1 cells. Enrichment of the promoter region measured by QRT-PCR. Bar graphs show mean ± sem. *, P < 0.05 as determined by ANOVA. B, Recruitment of GR and ERα to the GILZ TSS was examined by ChIP after treatment with vehicle, 100 nm Dex, 10 nm E₂, or 100 nm Dex and 10 nm E₂. Enrichment measured by primers directed to the TSS and quantified by QRT-PCR. Bar graphs show mean ± sem. *, P < 0.05 as determined by ANOVA. C, Recruitment of activated Pol II to the TSS of GILZ determined by ChIP after treatment of ECC1 cells with vehicle, 100 nm Dex, 10 nm E₂, or 100 nm Dex and 10 nm E₂ for 1 h. Enrichment measured by QRT-PCR. Bar graphs show mean ± sem. The symbols represent values that are statistically different at P < 0.01 from one another as determined by Tukey's post hoc analysis after ANOVA. D, Recruitment of GR to a NFκB site in IL-8 and ERα to an ERE in TFF1 were analyzed by ChIP and quantified by QRT-PCR for control. Bar graphs show mean ± sem. **, P < 0.01 as determined by ANOVA.