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Triple immunofluorescence labeling of β2-subunit of nAChR, M4 subtype of mAChR, and LH in anterior pituitary sections. A, β2-subunit (green fluorescence). B, M4 subunit (red fluorescence). C, LH-containing cells (blue fluorescence). D, Overlay, both β2-subunit and M4 subunit were observed in LH-containing cells. Nuclei were devoid of staining in all cases. Scale bar, 20 μm.
