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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Pathol. 2012 Jun 6;228(2):170–180. doi: 10.1002/path.3992

Figure 6.

Figure 6

Alveolar and exudate lung macrophages express PAI-1. (A–C) Diphtheria toxin (10.0 μg/kg) was administered for 14 days/protocol to DTR− (n = 3) and DTR+ mice (n = 3). Lung leukocytes from each strain of mice were isolated, pooled and antibody stained as described in Materials and methods. Multi-parameter fluorescence-activated cell sorting was performed to eliminate non-macrophage populations and to further isolate two populations of autofluorescent lung macrophages: CD11c+ CD11b− alveolar macrophages and CD11c+ CD11b+ exudate macrophages. (A, B) Photomicrographs (taken with a ×1000 objective) of sorted alveolar macrophages (A) and exudate macrophages (B). RNA was harvested from each macrophage subset and PAI-1 mRNA expression was assessed via qRT–PCR and normalized to GAPDH (C). Light grey bars, WT/DT; medium grey bars, DT/DTR+.