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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Pathol. 2012 Jun 6;228(2):170–180. doi: 10.1002/path.3992

Figure 7.

Figure 7

PAI-1 mediates the accumulation of exudate macrophages and Ly-6Chigh monocytes following alveolar injury. Diphtheria toxin (10.0 μg/kg) was administered for 14 days/protocol to three strains of mice: 1, DTR−: PAI-1+/+; 2, DTR+: PAI-1+/+; and DTR+: PAI-1−/−. Lung leukocytes from individual mice were isolated and antibody stained. Multi-parameter flow cytometric analysis was used to identify and enumerate total numbers of alveolar macrophages (A), exudate macrophages (B) and Ly-6Chigh monocytes (C). Data are given as mean ± SEM of nine DT-treated mice/strain (from two separate experiments), assayed individually; light grey bars, DT/WT; medium grey bars, DT/DTR+; and dark grey bars, DT/DTR+/PAI-1−. Values of p < 0.05 compared by unpaired Student’s t -test between groups at the same time point were considered significant.