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(A) Fluorescence in situ hybridization demonstrating osteosarcoma tumor with EGFR gene amplification (green signal: EGFR test probe at 7p11.2; red signal: control probe at 7q31.2). (B) Another osteosarcoma tumor harboring biallelic loss of PTEN (red signal: PTEN test probe at 10q23.21; green signal: control probe at 10p11.21); note the presence of chromosome 10 polysomy.
