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. 2012 Dec 24;199(7):1083–1101. doi: 10.1083/jcb.201202126

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© 2012 Schmidt et al.

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Figure 7. — Cep164-C interacts with Rabin8, but not with Rab8, in vitro. (A–C) In vitro binding assays using bacterially purified proteins. (A) MBP or MBP-Cep164-C beads were incubated with purified 6His-Rabin8. (B) MBP, MBP-Rabin8, or MBP-Cep164-C beads were incubated with NusA-Rab11a-6His. (C) MBP-fusion proteins (immobilized on beads) were incubated with purified NusA-Rab8a-T22N-6His. Proteins were visualized by Western blot. (D) Coomassie blue–stained gel of proteins used in A–C. The asterisks indicate unspecific protein bands.