TABLE 1.
Bacterial species and strain |
Structural feature |
Proposed elastic deformation model |
E* (kPa)b calculated by the Hertz model |
||
---|---|---|---|---|---|
h0 (nm)b |
S0 (104 nm2) |
E* (kPa)b | |||
S. epidermidis | |||||
ATCC 35983 | Poor slime producer | 11 ± 2 | 1.8 | 22 ± 4 | 7,190 ± 1,718 |
ATCC 35984 | Strong slime producer | 53 ± 7 | 8.3 | 8 ± 2 | 510 ± 160 |
S. salivarius | |||||
HB-C12 | Nonfibrillated | 28 ± 3 | 4.4 | 13 ± 2 | 1,320 ± 254 |
HB-7 | Fibrillated (91 nm long) | 44 ± 1 | 6.9 | 7 ± 1 | 593 ± 151 |
S. aureus | |||||
NCTC 8325-4 | Wild-type | 12 ± 2 | 2.0 | 47 ± 26 | 5,472 ± 3,020 |
NCTC 8325-4 Δpbp4 | Deficient in peptidoglycan cross-linking | 91 ± 2 | 14.2 | 10 ± 1 | 170 ± 62 |
Structural features of the different pairs of bacterial strains included in this study together with dimensions and reduced Young’s moduli of the contact cylinder between adhering bacteria and a glass substratum. The reduced Young’s moduli were obtained from the proposed elastic deformation model of the contact cylinder and from Hertz modeling of the compression data, pertaining to the soft, outermost cell surface and for an unknown part to the bacterial hard core (Fig. 1).
The values for h0 and E* are means ± standard deviations for deformation measurements taken over 24 different spots on the glass substratum, comprising eight different bacteria.