TABLE 2 .
Susceptibilities of B. subtilis bactobolin-resistant mutants to bactobolin A, bactobolin B, and other antibiotics
| Mutationb | MIC (µg per ml)a |
||||||
|---|---|---|---|---|---|---|---|
| Bact A | Bact B | Cm | Clin | Ery | Kan | Lin | |
| None | 3 (1) | 50 | 7 (5) | 4 (2) | 0.7 (0.6) | 2 (1) | 2 (3) |
| E236A | 17 (6) | >200 | 5 (2) | 3 (3) | 0.3 (0.1) | 2 (1) | 1 (1) |
| 235Gc | 100 | >200 | 3 (1) | 2 (1) | 0.3 (0.3) | 2 (1) | 1 (0.2) |
| E236Q | 21 (6) | >200 | 5 (2) | 4 (3) | 0.3 (0.1) | 2 (1) | 1 (1) |
a
MICs were determined with three independent experiments, and the range is indicated in parentheses. Antibiotics are bactobolin A (Bact A), bactobolin B (Bact B), chloramphenicol (Cm), clindamycin (Clin), erythromycin (Ery), kanamycin (Kan), and linezolid (Lin).
b
Strains shown are wild-type B. subtilis 3610 and the bactobolin-resistant mutants of 3610 with the indicated amino acid changes in L2 (encoded by rplB), corresponding with the following base changes (in order of appearance in the table): A708C, 706GGT, and G706C.
c
Two 235G variants were initially identified, and results with the two were identical.