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. 2012 Jul 12;33(10):1953–1964. doi: 10.1093/carcin/bgs225

Fig. 4.

Fig. 4.

Effect of MUC4 knockdown on key signaling molecules in PC cells (A and B) involved in apoptosis (caspase 9), metastasis and invasion (pScr, phospho and total FAK, phospho and total Akt and phospho and total ERK1/2, NFkB, FGFR1, MMP-9) in Capan1/BxPC3-shMUC4 and Capan1/BxPC3-Scr vector cells. The results showed upregulation of Caspase-9 and downregulation of pSrc, pFAK (Tyr576/577, Tyr925), pERK1/2, PAkt, NFkB, FGFR1 in Capan1/BXPC3-shMUC4 compared with scramble vector transfected cells. The total form of FAK, Akt and ERK molecules remains unchanged. β-actin was used as a loading control. (C) Reciprocal co-immunoprecipitation analysis to show the interactions between N-cadherin and FGFR1. Lysates from the MUC4-expressing Capan1/BxPC3 cell lines were utilized for immunoprecipitation with N-cadherin and FGFR1 antibodies. The immunoprecipitates were electrophoretically resolved on 10% polyacrylamide gel and immunoblotted with anti-N-cadherin or anti-FGFR1 antibodies. The isotype antibodies were used as controls. (D) Real-time PCR analysis using primers that specifically amplify the FGFR1 and N-cadherin genes showed reduced expression of FGFR1 and N-cadherin in MUC4 knockdown Capan1and BxPC3 cells lines were utilized for immunoprecipitation with N-cadherin and FGFR1 antibodies.