Fig. 2.
Analysis of the [35S]sulfated products generated and released by HepG2 human hepatoma cells labelled with [35S]sulfate in the presence of ractopamine or salbutamol. The autoradiograph taken from the TLC plate used for TLC analysis of the labelling media is shown. Confluent HepG2 cells were labelled with [35S]sulfate for 18 h in the presence of different concentrations (5 or 50 µM) of ractopamine and salbutamol. Lane 1 shows the control labelling medium without added compound. Lanes 2–5 correspond to the labelling media containing 5 or 50 µM ractopamine and 5 or 50 µM salbutamol, respectively. The figure is representative of three independent experiments.