. Author manuscript; available in PMC: 2014 Jan 31.

Published in final edited form as: J Immunol Methods. 2012 Oct 23;387(1-2):317–321. doi: 10.1016/j.jim.2012.10.011

Table 1.

Preparation of the known molar ratio of protein mixture

Protein 1	Protein	Conc. of protein 1 (mg/mL)	Conc. of protein 2 (mg/mL)	Volume of protein 1 in mixture (μL)	Volume of protein 2 in mixture (μL)	% Mass of Protein 1^a	% Mass of Protein 2^b	Molar ratio of protein 1/protein 2^c
Pfs25	BSA	0.770	0.752	37.5	12.5	75%	25%	1.111
				25	25	51%	49%	3.332
				12.5	37.5	25%	75%	9.995

AMA1-FVO	EPA	0.437	0.841	5	15	15%	85%	0.187
				10	10	34%	66%	0.562
				15	5	61%	39%	1.685

Pvs 25	EPA	0.690	1.265	15	10	45%	55%	2.650
Pvs28	EPA	1.308	1.225	10	10	52%	48%	3.396

$\begin{array}{l} % Mass % of protein 1 = [(volume of protein 1 in the mixture \times concentration of protein 1) \div (volume \\ of protein 1 in the mixture \times concentration of protein 1 + volume of protein 2 in the mixture \times \\ concentration of protein 2)] \times 100 \end{array}$

$\begin{array}{l} % Mass of protein 2 = [(volume of protein 2 in the mixture \times concentration of protein 2) \div (volume of \\ protein 1 in the mixture \times concentration of protein 1 + volume of protein 2 in the mixture \times concentration \\ of protein 2)] \times 100 \end{array}$

$\begin{array}{l} Molar ratio of protein 1 / protein 2 = (% Mass of protein 1 \div molecular weight of protein 1) \div (% Mass of \\ protein 2 \div molecular weight of protein 2) \end{array}$