Table 3.

Comparison of the nominal molar ratio and the calculated molar ratio

Protein 1/Protein 2	Molar ratio of protein 1/protein 2 (nominal)a	Molar ratio of protein 1/protein 2 (calculated)b		% Accuracyc
		By intact MWd	By modified MWe	By intact MWd	By modified MWe
Pfs25/BSA	1.111	1.016	1.072	91.49	96.52
	3.332	3.022	3.187	90.71	95.67
	9.995	9.731	10.267	97.36	97.27
AMA1-FVO/EPA	0.187	0.201	0.197	92.63	94.69
	0.562	0.584	0.573	95.96	97.95
	1.685	1.646	1.614	97.65	95.77
Pvs 25/EPA	2.650	2.422	2.628	91.41	99.18
Pvs28/EPA	3.396	3.370	3.195	99.24	94.07
		Average % Accuracy		94.55	96.39
		Standard deviation		3.36	1.70

^aKnown molar ratio of protein 1 to protein 2 (nominal) was obtained by preparing the known molar concentration of protein 1 and protein 2 in the same mixed sample.

^bMolar ratio of protein 1 to protein 2 (calculated) was calculated by the method presented in Table 2 using the amino acid data.

^c $\begin{array}{l}\%\text{Accuracy}=[100\%-\mid (\text{molar}\text{ratio}\text{of}\text{calculated}-\text{molar}\text{ratio}\text{of}\text{nominal})/(\text{molar}\text{ratio}\text{of}\\ \text{nominal})\mid ]\times 100\%\end{array}$.

^dIntact molecular weight of protein 1 and protein 2 were used in the calculation.

^eModified molecular weight of protein 1 and protein 2, i.e. sum of molecular weight of amino acid residues actually used in the calculation were used in the calculation.