Table I. Parameters derived from a log (mAb concentration) versus response (Young's modulus, E) equation.
| Ab | E∞/E0a | C50b | GXM Affinityc |
|---|---|---|---|
| 18B7 (IgG1) | 24.8 | 6.3 | 0.1 |
| 2D10 (IgM) | 10.8 | 1.7 | 0.4 |
| 13F1 (IgM) | 2.3 | 11.4 | 9.5 |
| 3E5 (IgG1) | 5.1 | 1.8 | + |
| 3E5 (IgG2a) | 6.3 | 8.6 | ++ |
| 3E5 (IgG2b) | 6.5 | 1.1 | +++ |
| 3E5 (IgG3) | n.d. | n.d. | + |
a
E values of the plateau region in relation to the initial (control) E values.
b
Concentration of mAb that results in E values halfway between the basal (control) and the maximal (plateau) values.
c
Dissociation constant (Kd) values for 18B7, 2D10, and 13F1 mAbs, using cryptococcal GXM from a 2-d culture of C. neoformans H99 cultures, were taken from Ref. 9. The + symbols represent the intensities of the relative affinities of the 3E5 switch variants, based on binding curves published in Ref. 39.
n.d., Not detectable.