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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Steroids. 2012 Nov 2;78(1):15–25. doi: 10.1016/j.steroids.2012.10.002

Figure 6. Competitive binding of DHEA metabolite to ERα and ERβ.

Figure 6

An ligand binding competition assay using [3H] E2 was performed using baculovirus expressed ERα (A) or ERβ (B) with increasing concentrations of nonradiolabeled E2, DHEA or DHEA metabolites (DHEA-S, 7-oxo-DHEA, ADIONE and ADIOL). The values on the Y-axes are expressed as the percentage of [3H] E2 bound and each data point represents the mean of two independent binding assays. The competitor concentration causing 50% reduction in [3H] E2 binding (IC50) is found at the intersection of the binding curves with the 50% binding line (---------). E2, ●; DHEA, ▲; DHEA-S, ♦; ADIOL, ■; ADIONE, ♦; and 7-oxo-DHEA, □.