Figure 2. MARCO is not expressed on the effector cells recruited to the nasopharynx.
(A) QRT-PCR on RNA lysis buffer nasal lavages at 30 mins (black bars), 3 days (grey bars) and 7 days (white bars) post-inoculation with P1121. Bars represent relative mRNA levels (GAPDH/Target) n ≥ 3. (B) Upper panels: Immunofluorescent staining of cytospin preparations from nasal lavages. Representative images from day 3. Images at 400× magnification. Inset in MARCO panel demonstrates positive staining in alveolar macrophages. Lower panels: Fresh frozen spleen sections stained as positive controls for scavenger receptor antibodies. Images at 100× magnification.
