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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: J Immunol. 2012 Nov 28;190(1):278–284. doi: 10.4049/jimmunol.1201667

Figure 1.

Figure 1

Generating and assessing cell wall extracts. (A) Scheme for generating cell wall extract by treating bacterial pellets with organic solvents chloroform (CHCl3) and methanol (CH3OH). T-cell clones (B) F5 or (C) LDN5 were co-incubated with monocyte-derived dendritic cells and titrating concentrations of either cell wall extract or whole ESAT-6 protein. Data are representative of three independent experiments.