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Cellular requirements for the production of IFN-γ by cell wall extracts. (A) PBMC from a subject with latent tuberculosis infection, DCs, and cell wall extract were tested in combination for the ability to induce IFN-γ production by ELISPOT. (B) FACS sorting strategy for PBMC. (C) Lymphocyte sub-populations were co-incubated overnight in the presence of 5 µg/ml cell wall extract and DCs prior to ELISPOT.
