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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: J Immunol. 2012 Nov 30;190(1):447–457. doi: 10.4049/jimmunol.1201641

FIGURE 2. Representative spectratype histograms in the B10.BR anti-CBA and anti-MMC6 CD8+Vβ13+ T cell responses in lymphoid tissue.

FIGURE 2

Spectratype analyses were performed on CD8+ T cell populations as described in Materials and Methods. (A) CD8+ splenic T cells harvested from naïve B10.BR mice acted as the control group. (B) For alloreactive responses (anti-CBA), CBA mice were exposed to lethal irradiation (11 Gy, split dose) and transplanted with 1-2×107 B10.BR CD8+ T cells. Splenocytes were harvested at 10-13 d post-BMT. (C) For the anti-tumor response (anti-MMC6), B10.BR mice were presensitized (i.p.) with 1×107 MMC6 tumor cells (40 Gy), followed by a second injection of 1×106 MMC6 cells (40 Gy) in the footpad 17-21 d later. After 1 wk, draining lymph nodes were harvested and CD8+ T cell isolated. Skewing was defined as a peak area greater than the mean of the control plus 5×SD. CDR3 lengths are shown under each peak. Skewed peaks are marked with an arrow.