Table I.
Comparison of the skewed CDR3-size lengths of B10.BR CD8+ T cells from the spleens of CBA recipients or MMC6 tumor presensitized B10.BR micea
| Vβ | α-CBA | α-MMC6 |
|---|---|---|
| 1 | — | 186, 189 |
| 2 | — | — |
| 3 | 149 | — |
| 4 | — | — |
| 5 | 172 | — |
| 6 | — | 138 |
| 7 | 176 | 167, 176 |
| 8.1 | — | — |
| 8.2 | 134 | 153 |
| 8.3 | 155, 170 | — |
| 9 | 147 | — |
| 10 | — | — |
| 11 | 157 | — |
| 12 | 201 | — |
| 13 | — | 160, 163, 166 |
| 14 | 160 | — |
| 15 | 182 | 182 |
| 16 | — | — |
| 18 | 225 | — |
| 20 | 196 | — |
For the anti-CBA response, lethally irradiated CBA recipient mice were injected with 1 × 107 B10.BR CD8+ T cells and spleens were harvested after 10 days. For the anti-MMC6 response, B10.BR mice were injected i.p. with irradiated MMC6 cells followed by footpad boost 3 weeks later. One week later, CD8+ T cells were isolated from inguinal, mesenteric, and popliteal draining lymph nodes. CDR3-size spectratype analysis was performed as described in Materials and Methods. Skewing of bands was defined as an average peak area greater than the average control area plus 5×SD. —, No skewing; CDR3 lengths are shown for skewed Vβ families. Results were averaged from 5 separate experiments.