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. Author manuscript; available in PMC: 2014 Feb 27.
Published in final edited form as: Cancer Lett. 2012 Aug 27;334(1):101–108. doi: 10.1016/j.canlet.2012.08.026

Fig. 4. Overexpression of Survivin inhibits WA-induced apoptosis in MDA-MB-231 and MCF-7 cells.

Fig. 4

(A) Immunoblotting for Survivin using lysates from MDA-MB-231 and MCF-7 cells stably transfected with empty vector (pCMV6-AC-GFP) or the same vector encoding for Survivin and treated for 24 h with DMSO or WA. The number above the immunoreactive band represents change in protein level relative to empty vector-transfected cells treated with DMSO (first lane from the left). (B) Quantitation of apoptosis by DAPI assay (MDA-MB-231 cells) or histone-associated DNA fragment release into the cytosol assay (MCF-7) in empty vector-transfected control cells and Survivin-overexpressing cells after 24-h treatment with DMSO or WA. Results shown are mean ± SD (n = 3). Significantly different (P<0.05) compared with aDMSO-treated empty vector-transfected cells and bbetween groups at each dose by one-way ANOVA followed by Bonferroni’s test. (C) Immunoblotting for PARP cleavage and cleaved caspase-3 using lysates from cells stably transfected with empty vector or vector encoding for Survivin and treated for 24 h with DMSO or WA. Quantitation relative to empty vector-transfected cells treated with DMSO is shown. In MDA-MB-231 cells, the antibody used recognized both full-length and cleaved forms of the PARP protein. An antibody specific for detection of cleaved PARP only was used for the immunoblotting using lysates from MCF-7 cells. The arrows indicate the cleaved form of PARP or caspase-3.