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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Exp Parasitol. 2012 Oct 29;133(1):44–50. doi: 10.1016/j.exppara.2012.10.011

Fig. 1.

Fig. 1

Relationship of the ratios of parental parasites and oocyst counts in genetic crosses. A) Green fluorescent parasite (GFP) -BY265G- producing few oocysts; BY265G was crossed with non-fluorescent N67 (B–D) or with NSM (E) parasites at 5:1 (B), 20:1 (C), and 10:1 (D and E) ratios. (F) A ratio of 10:1 consistently produced more green oocysts from day 7 to day 14; standard deviation bars were from at least 10 mosquitoes. Crossing BY265G with N67 or NSM can greatly improve the efficiency of green oocyst production by BY265G, suggesting gain of genetic elements from the N67 or NSM during the crosses. In this case, the majority of green oocysts should be recombinant containing BY265 genetic background with the gene expressing GFP and genes that are necessary for producing large numbers of oocysts.