Table 4.

Numbers of infected mice and recombinant progeny from genetic crosses cloned at different ratios of parental genotypes or parasitemia.

				Number(%)
Experiments	Parasitemia	No. Mice	No. Inf. Mice	NSM(%)	BY265(%)	MG(%)	RP(%)	IRP(%)
1	0.27%	50	6	5 (83.3)	0(0)	1(16.7)	0(0)	0(0)
2	0.57%	46	12	2(16.7)	0(0)	1(8.3)	9(75.0)	4(33.0)
3	0.74%	51	30	20(66.7)	0(0)	5(16.7)	5(16.7)	3 (10.0)
4	6.22 %	50	29	27(93.1)	0(0)	2(6.9)	0(0)	0(0)

Note: Experiment 1 and 2 were cloned based on different intensity of PCR bands on agarose gels. Parasite mixture in experiment 1 produced similar band intensity for both parental alleles (Fig. 2A, lane 4), whereas experiment 2 had a stronger band for BY265G (Fig. 2D, lane 1). Experiment 3 and 4 were cloned (at 1.3 infected red blood cells per mouse) from a mouse injected with infected blood from another cross at different parasitemia. . Recombinant progeny were identified using 10 microsatellite loci (Py1618, Py2673, Py1428, Py1404, Py2311, Py1352, Py2000, Py2857, Py2469, Py1318) as described previously (Li et al., 2011). No. Mice, numbers of mice used in the cloning experiments; No. Inf. Mice, numbers of infected mice; NSM(%), numbers and percentage of progeny with NSM type; BY265(%), number and percentage of BY265 type; MG(%), numbers and percentage of mixed genotype; RP(%), number and percentage of recombinant progeny; IRP(%), numbers and percentage of independent recombinant progeny.