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. 2012 Nov 20;137(4):326–335. doi: 10.1111/j.1365-2567.2012.03622.x

Figure 4.

Figure 4

The effect of interleukin-27 (IL-27) in interferon-γ (IFN-γ)-deficient mice. After primary immunization, IFN-γ knockout (KO) mice were rapidly injected intravenously with 100 μg IL-27-Fc in 2 ml saline within 5 seconds. After 8 and 10 days, the mice were injected intramuscularly with 100 μg IL-27-Fc in the left and right leg, respectively, by electroporation. The (a) clinical score and (b) incidence rate of disease is shown for both collagen-induced arthritis (CIA) and IL-27-Fc-treated mice over time. Data are representative of two independent experiments with five per group (error bars, SD). (c) The concentrations of type II collagen (CII)-specific IgG and IgG2a were determined by ELISA. (d) Images show the degree of inflammation and cartilage damage based on haematoxylin & eosin, toluidine blue and safranin O staining of the tibia–talus joint in the ankle of CIA and IL-27-Fc-treated mice. Scale bars, × 40: 500 μm, × 200: 500 μm.