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. 2012 Dec 3;171(1):91–99. doi: 10.1111/j.1365-2249.2012.04676.x

Fig. 1.

Fig. 1

Altered expression of microRNA (miRNA) in T cells from patients with systemic lupus erythematosus (SLE). (a) Expression profile of 270 microRNAs (miRNAs) in T cells from SLE patient and healthy controls measured by real-time polymerase chain reaction (PCR). Each scatter spot represents the average normalized expression level in T cells from five SLE patients and five healthy controls for each miRNA. The threshold cycle (Ct) is defined as the cycle number at which the change of fluorescence intensity crosses the average background level of the fluorescence signal. The normalized miRNA level was defined as (39 – Ct) after normalization with the internal control (U6 small nuclear RNA). All data are presented as mean ± standard deviation. (b) Seven miRNAs were found to be potentially aberrant expressions in SLE T cells. miR-145, miR-513-5p, miR-150, miR-516a-5p, miR-483-5p and miR-629 were down-regulated, whereas miR-224 was up-regulated (fold change > 3·9; P-value < 0·05). (c) Altered expression of miR-145 and miR-224 in T cells from SLE patients after validation. The seven abnormally expressed miRNAs in T cells were validated by real-time PCR from 21 SLE patients and 22 healthy controls.