Fig. 3.

Effects of microR (miR)-145 transfection on signal transducer and activator of transcription-1 (STAT)-1 gene expression in Jurkat cells after electroporation. The expression of mRNA, miRNAs and protein was analysed 24 h after transfection. (a) The expression level of miR-145 was elevated dramatically in the Jurkat cells after transfection with miR-145 compared to the scramble oligonucleotide-transfected control groups. (b) The expression levels of STAT-1 mRNA were suppressed modestly but significantly by miR-145 transfection. (c) The STAT-1 protein expression was suppressed significantly by miR-145 transfection after 24 h. (d) A representative example.