Fig. 4.

Effects of microR (miR)-224 transfection on apoptosis inhibitory protein 5 (API)5 expression in Jurkat cells by electroporation. The expression of mRNA, miRNAs and protein was analysed 24 h after transfection. (a) The expression level of miR-224 was elevated dramatically in the Jurkat cells after transfection with miR-224 compared to the scramble oligonucleotide-transfected control groups. (b) After transfection for 24 h, the expression levels of API5 mRNA were not suppressed significantly by miR-224 transfection. (c) The expression of API5 protein was suppressed significantly by miR-224 transfection. (d) A representative example.