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Effects of microR (miR)-224 transfection on activation-induced cell death in Jurkat cells by electroporation. The miR-224 or scramble RNA-transfected (control) Jurkat cells were then stimulated with ionomycin (500 ng/ml) and phorbol myristate acetate (PMA) (10 mg/ml) for 24 h. The treated transfected Jurkat cells were then stained by propidium iodide (PI) staining solution for measurement of % apoptosis by flow cytometry. (a) A representative case; (b) percentage of cell apoptosis.
