Table 1.
Primer sets used for RT-qPCR
Gene | Primers (5′ to 3′) | Amplicon (bp) |
---|---|---|
NIS (r) | Forward GCTGTGGCATTGTCATGTTC | 270 |
Reverse TGAGGTCTTCCACAGTCACA | ||
NIS (m) | Forward AGCTGCCAACACTTCCAGAGG | 145 |
Reverse GCTGATGAGAGCACCACAAAG | ||
ALP (r) | Forward CTGCTGAGCAGGAATCCACA | 364 |
Reverse TAGTTGACATCACCACTCTC | ||
ALP (m) | Forward TGGCTGTCAAAGCATCAGGGA | 146 |
Reverse TCTACTGAGGGGTTTCGGTTGG | ||
β-Actin (r) | Forward GGCACCACACTTTCTACAATG | 138 |
Reverse TGGCTGGGGTGTTGAAGGT | ||
β-Actin (m) | Forward CAGCCTTCCTTCTTGGGTATGG | 145 |
Reverse CAGCAATGCCTGGGTACATGG | ||
18S | Forward GGCCTGCGGCTTAATTTGAC | 243 |
Reverse GTGGCTGAACGCCACTTGTC | ||
Neo | Forward ACAATCGGCTGCTCTGATG | 260 |
Reverse GATACTTTCTCGGCAGGAGC | ||
GFP | Forward ACCACATGAAGCAGCACGAC | 93 |
Reverse TGTAGTTGCCGTCGTCCTTG |
The species of origin for primer sequences are indicated in parentheses following each primer pair (m, mouse; and r, rat).