Skip to main content
. 2012 Sep 10;590(Pt 23):6121–6139. doi: 10.1113/jphysiol.2012.240986

Figure 2. Localization of pS124-NCC in Munich–Wistar rat kidney.

Figure 2

A, using immunohistochemistry, anti-pS124-NCC antibody shows labelling of all DCT segments. B, higher magnification revealing distinct pS124-NCC labelling of the apical membrane of DCT cells. C, confocal laser scanning micrograph demonstrating that pS124-NCC (green) colocalizes with total NCC (red). D and E, pS124-NCC (green) partially colocalizes with calbindin (red), a late DCT and connecting tubule (CNT)-specific marker. F, pS124-NCC (green) does not colocalize with the CNT and collecting duct principal cell-specific marker, aquaporin-2 (red). G, double immunogold electron micrograph demonstrating total NCC (small arrows) and pS124-NCC (large arrows) in the apical domain of DCT cells. Labelling was associated with the apical plasma membrane (upper left panel), but also intracellular vesicles and the Golgi apparatus (lower right panel). Gold particle diameter: 5 nm (pS124-NCC, large arrows) and 15 nm (total NCC, small arrows).