Table 2.
[33P]ATP kinase assays demonstrate weak activity of selected kinases on a synthetic peptide corresponding to the region surrounding S124 of NCC
| Protein kinase | Control substrate | % Activity against ETGANSEKSPGEPVR |
|---|---|---|
| ETGANSEKSPGEPVR at 10 μm | ||
| ERK5 | Myelin basic protein | 1 |
| NLK | Myelin basic protein | 9* |
| p38δ | Myelin basic protein | 2 |
| ERK2 | Myelin basic protein | 3 |
| mTOR/FRAP | 4EBP1 | 9* |
| p38γ | Myelin basic protein | 2 |
| ETGANSEKSPGEPVR at 250 μm | ||
| AKT2 | AKT (SGK) synthetic peptide | 7* |
| AKT3 | AKT (SGK) synthetic peptide | 4 |
| AMPK(A1/B1/G1) | SAMStide synthetic peptide | 7* |
| CDK4/Cyclin D3 | RB protein | 4 |
| CDK5/p38 | Histone H1 | 1 |
| CDK6/Cyclin D1 | RB protein | 10* |
| CDK6/Cyclin D3 | RB protein | 6* |
| GSK3α | GSK3 | 1 |
| HIPK3 | Myelin basic protein | 4 |
| IRAK2 | Modified AKT peptide | 13* |
| P70s6k | S6K synthetic peptide | 3 |
| PDK1 | PDKtide synthetic peptide | 6* |
| PKCζ | CREBtide synthetic peptide | 2 |
| RAF1 | Unactive MEK1 & ERK1 | 2 |
| ROCK1 | S6K synthetic peptide | 3 |
| RSK1 | S6K synthetic peptide | 1 |
| RSK3 | RSK synthetic peptide | 1 |
| ZAP70 | Poly (Glu/Tyr, 4:1) synthetic peptide | 2 |
The results are presented as the percentage activity change of the kinase against the synthetic S124-NCC peptide, compared to control substrate. *Significant activity (% > 5).