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. Author manuscript; available in PMC: 2013 Dec 21.
Published in final edited form as: Chem Biol. 2012 Dec 21;19(12):1600–1610. doi: 10.1016/j.chembiol.2012.10.013

Figure 6.

Figure 6

Major contacts between 29-membered thiopeptides and EF-Tu. (A) Crystal structure of GE2270A (dark blue sticks) complexed with T. thermophilus EF-Tu (green ribbon, E. coli numbering) and GDPNP (GTP analog, not visible) adapted from PDB: 2C77 (Parmeggiani, et al., 2006). Residues forming important contacts are illustrated in light blue. Residues involved in resistance mutations are noted in red: G275S occurs in B. subtilis G1674 used in this work. (B) EF-Tu contacts (black, E. coli numbering) identified from GE2270A-EF-Tu structures complexed with GDP or GTP and mapped onto GE37468 (blue). GE37468 residues in bold were subject to randomization. Contacts and references are tabulated in Table S1. Contact with residue Q98 from T. thermophilus EF-Tu is only formed in the GTP bound state.