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. Author manuscript; available in PMC: 2013 Dec 21.
Published in final edited form as: Cell. 2012 Dec 13;151(7):1457–1473. doi: 10.1016/j.cell.2012.11.026

Figure 7. Dasatinib impairs the proliferation of β-catenin active cell lines.

Figure 7

(A) Proliferation (7 d) following dasatinib treatment. Proliferation of (B) HCT116 or (C) HuTu80 cells following dasatinib treatment of cells stably expressing dasatinib resistant YES1 (T348I) or SRC (T341I) mutants. Data presented as mean ± SD for 4 independent experiments. (D) Representative images of colon organoids derived from WT or APC null mice treated for 6 d with 100 nM of dasatinib. (E) Quantification of results in D. Error bars represent SD from 4 replicates. Blank mark wells where no organoids were added. (F) WT or AXIN1 mutant (Masterblind) zebrafish were treated with 2 μM of dasatinib from 6-8 dpf. Arrow indicates developing gut. (G) H&E staining of zebrafish in (F). Width of epithelium noted by bars. (H) The number of epithelial cells/section was measured in WT or AXIN1 mutant zebrafish treated with 2 μM of dasatinib or DMSO. Error bars represent the SD from 20 different sections from 5 treated fish. P value was calculated using student’s t test.