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. Author manuscript; available in PMC: 2013 Dec 21.
Published in final edited form as: Cell. 2012 Dec 21;151(7):1608–1616. doi: 10.1016/j.cell.2012.11.018

Figure 2.

Figure 2

H2A.Z and Foxa2 are required for nucleosome depletion during ES cell differentiation

(A) Nucleosome distribution surrounding Foxa2 binding sites in ES and EHP cells. Note the decrease in nucleosome occupancy at Foxa2 sites in differentiated cells.

(B and C) Genome-wide distribution of nucleosome depletion or occupation regions near H2A.Z, H2A.X and Foxa2 binding sites.

(D) Co-binding regions of Foxa2 and H2A.Z overlap with nucleosome depletion regions. Foxa2 binding sites were pooled from EHP and pEHP (partially differentiated, see Figure S3A) cells; H2A.Z binding sites were pooled from ES and pEHP cells.

(E and F) Foxa2 and H2A.Z tag density near Foxa2/H2A.Z-associated nucleosome depletion regions.

(G) Co-localization of Foxa2/H2A.Z and nucleosome depletion regions in the intron of the Foxa1 gene.

(H) Nucleosome depletion during ES-cell differentiation is dependent on Foxa2 and H2A.Z. Nucleosome occupancy was determined by qPCR at ten nucleosome depletion regions that are bound by both H2A.Z and Foxa2 in differentiated ES cells. Relative nucleoscome occupancy is shown for ES cells, sorted EHP cells, un-sorted EHP (siRNA (−), scramble siRNA control) cells, and un-sorted EHP cells transfected with siRNAs for H2afz (H2A.Z) or Foxa2. Error bars represent standard error of the mean.

See also Figures S2–S5.