Foxa2/H2A.Z-driven nucleosome depletion complexes during ES cell differentiation. (A–C) Smarca4 (Brg1), Kat5 (Tip60), and Nap1l1, are enriched at nucleosome depletion regions. The tag density was normalized to 10 million sequencing tags for each sample; bin = 1 bp. (D) Immunoprecipitation (IP) experiments were performed with differentiated EHP cells using anti-H2A.Z or anti-Foxa2 antibodies and IP complexes were detected using western blotting with antibodies against Foxa2, H2A.Z, Smarca4, Kat5 and Nap1l1. Anti-IgG antibodies were used as negative IP controls.
See also Figures S2–S5.