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. Author manuscript; available in PMC: 2013 Dec 21.
Published in final edited form as: Cell. 2012 Dec 21;151(7):1608–1616. doi: 10.1016/j.cell.2012.11.018

Figure 4.

Figure 4

Nucleosome dynamics and ES cell differentiation. Flow cytometry analysis with dual cell surface markers of Foxa2/CD4 and ENDM1 for the assessment of the extent of differentiation

(A) Control, differentiated ES cells sorted without incubation with primary antibodies.

(B) Undifferentiated ES cells sorted with both antibodies. Less than 1% of the cells are double positive.

(C) Differentiated ES cells, with more than 40% double-positive cells.

(D) ES cells treated with siRNA to H2afz show decreased differentiation potential.

(E) ES cells treated with siRNA to Foxa2 show decreased differentiation potential.

(F) ES cells treated with 100 μM RG108, a DNA methyltransferase inhibitor, exhibit decreased differentiation.

See also Figure S5.