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. Author manuscript; available in PMC: 2013 Dec 20.
Published in final edited form as: Neuron. 2012 Dec 20;76(6):1189–1200. doi: 10.1016/j.neuron.2012.10.036

Figure 1. Generation and characterization of Cre and floxed-diphtheria toxin receptor lines.

Figure 1

(A–C) Generation of mossy cell/CA3-Cre transgenic line #4688. (A) A parasagittal section stained with X-gal and Safranin O from the brain of Cre mice crossed with loxP-flanked Rosa26LacZ (8-wk old) reporter line (arrow, ventral hippocampus). (B) Cre-IR in the dentate gyrus of (8-wk old) Cre mice. Note that Cre is expressed selectively in the dentate hilus (arrowhead) but not in area CA3c. (C) Confocal immunostaining images of β-gal (green, recombination product) with calretinin (red, mossy cell marker) or GluA2/3 (red, mossy cell marker), and β-gal (red) with GAD67 (green, GABAergic interneuron marker), of Cre/Rosa26LacZ double transgenic mouse show more than 90% of calretinin- or GluA2/3-positive cells are β-gal positive but no colocalization of β-gal with GAD67. (D) Alkaline phosphatase-stained parasagittal section from the brain of (8-wk old) floxed-diphtheria toxin receptor (fDTR) line-B.