(A) Timm staining of septal and temporal hippocampi during the chronic phase after mossy cell loss. Mutants show no mossy fiber sprouting and mossy cell axon terminal band-like staining in IML (arrowheads) almost disappears.
(B) ZnT3-IR in mutants shows no mossy fiber sprouting and loss of mossy cell axon terminal band-like staining in IML (see arrows in control).
(C) (Left) Hippocampal GAD67-IR during chronic phase. (Right) The sprouting score (modified Tauck and Nadler method) is higher in mutant IML than in controls (arrowheads). Mann-Whitney U-test (*p<0.05).
(D) Representative 2 hr traces of in vivo LFP recorded from mutant mouse before treatment (untreated) and 7 days (acute phase) and 5 weeks (chronic phase) after DT treatment. Epileptic LFP events following KA injection (20 mg/kg i.p.) to wild-type mouse are also shown. No obvious epileptiform discharges were observed following DT treatment.