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. Author manuscript; available in PMC: 2013 Dec 20.
Published in final edited form as: Neuron. 2012 Dec 20;76(6):1189–1200. doi: 10.1016/j.neuron.2012.10.036

Figure 6. Functional changes after mossy cell loss.

Figure 6

(A) Timm staining of septal and temporal hippocampi during the chronic phase after mossy cell loss. Mutants show no mossy fiber sprouting and mossy cell axon terminal band-like staining in IML (arrowheads) almost disappears.

(B) ZnT3-IR in mutants shows no mossy fiber sprouting and loss of mossy cell axon terminal band-like staining in IML (see arrows in control).

(C) (Left) Hippocampal GAD67-IR during chronic phase. (Right) The sprouting score (modified Tauck and Nadler method) is higher in mutant IML than in controls (arrowheads). Mann-Whitney U-test (*p<0.05).

(D) Representative 2 hr traces of in vivo LFP recorded from mutant mouse before treatment (untreated) and 7 days (acute phase) and 5 weeks (chronic phase) after DT treatment. Epileptic LFP events following KA injection (20 mg/kg i.p.) to wild-type mouse are also shown. No obvious epileptiform discharges were observed following DT treatment.