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. Author manuscript; available in PMC: 2012 Dec 26.
Published in final edited form as: J Clin Cell Immunol. 2012 Jul 16;3(3):124. doi: 10.4172/2165-8048.1000124

Fig. 2. Effect of NO modulators on the encephalitogenicity of MBP-specific T cells.

Fig. 2

A) Lymph node cells (LNC) isolated from MBP-immunized donor female SJL/J mice were re-primed with MBP in the presence of either PTIO (50µM), or DETA-NONOate (DNO; 100µM). After 4 days, viable non-adherent cells (2 × 107) were adoptively transferred to naïve female SJL/J recipient mice via tail-vein injection. B) Donor mice were immunized with MBP as described under methods section, and on 2 and 8 day post immunization (dpi), mice were treated with either PTIO (100mg/kg) or saline as vehicle via i.p. injection. On 12 dpi, mice were sacrificed, and LNC were further primed with MBP (50 µg/ml) for 4 days followed by transfer of viable non-adherent cells to naïve recipient mice. Six recipient mice (n=6) were used in each group. Mice were examined for clinical symptoms daily. Data are mean ± SEM of six mice per group.