On 14 dpt, spinal cord (A) and cerebellar (B) sections of HBSS-treated (normal), MBP-primed T cell-treated, PTIO-incubated MBP-primed T cell-treated, and DNO-incubated MBP primed T cell-treated mice were stained with Luxol fast blue. Digital images were collected under bright field setting. Demyelination in spinal cord (C) and cerebellum (D) was represented quantitatively by using a scale as described in materials and methods. Data are expressed as the mean ± SEM of five different mice per group. ap < 0.001 vs HBSS (normal); bp < 0.001 vs MBP-primed T cells; cp < 0.05 vs MBP-primed T cells. Spinal cord (E) and cerebellum (F) were analyzed for the mRNA expression of CNPase, MOG and PLP by semi-quantitative RT-PCR. Results represent the analysis of five different mice per group.