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Reversible vesicle growth driven by amphiphile concentration changes. Myristoleate vesicles, initially at 10 mM, shrink in surface area upon dilution to 5 mM. Surface area grows back to near the initial value upon concentration via the addition of preformed vesicles and subsequently shrinks upon further dilution. Changes in membrane area are tracked by FRET between donor and acceptor phospholipids, which remain in the vesicles due to their insolubility.
