Figure 5.

The resistance of the PSAR-20 brush surface (chain density = 0.87 nm⁻²) against 3T3 mouse fibroblast attachment. The cells were stained with calcein AM. (A) plots the surface coverage of fibroblasts observed on the PSAR-20 brush over 49 d (7 wk). Fresh fibroblasts were reseeded on the samples twice a week. The number of cells attached on PSAR-20 was very low throughout, while a confluent layer (coverage = 1) was achieved on the uncoated TiO₂ control surface after 7 d. Although some cells were observed on the PSAR-20 surface, (B) shows that their morphologies were rounded, exhibiting few filopodia, and were poorly attached. In contrast, cells on the TiO₂ control spread very well and formed a confluent cell sheet after 1 wk of cell culture (A). The error bars indicate ±1 SD.