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. 2012 Dec 26;7(12):e52647. doi: 10.1371/journal.pone.0052647

Figure 3. The protective effects of angiotensin II (AT-II) are induced rapidly.

Figure 3

(a) Hepatocytes were pre-incubated with 100 nmol/L of AT-II for 10 minutes after which cells were washed and exposed to 50 µmol/L of GCDCA alone (+ AT-II/washed) or with simultaneous addition of AT-II (+ AT-II). Two-way ANOVA test was used to determine the significance of differences between experimental groups (N = 4). * P<0.05 for GCDCA+AT-II/washed vs. GCDCA 50 µM and for GCDCA+AT-II vs. GCDCA 50 µM. (b) hepatocytes were stimulated with 50 µmol/L GCDCA for 4 hours (GCDCA 50). AT-II (100 nmol/L) was added 10 minutes prior to (−10 min), simultaneous with (0 min) or 30 minutes (+30 min), 1 hour (+1 hr), 2 hours (+2 hrs), 3 hours (+3hrs) after the addition of GCDCA. One-way ANOVA test was used to determine the significance of differences between experimental groups (N = 4). *P<0.05 for −10 min of AT-II, 0 min of AT-II, +30 min of AT-II vs GCDCA alone.