Figure 7. ena is required for rearrangement and cell shape changes of SGPs.

(A) The circularity of individual SGPs was calculated in the anterior, middle, and posterior regions of the gonad. Circularity (see Materials and methods) was similar between the wild type and mutants at stage 13. However, at stages 15–16, circularity increased with compaction in wild-type SGPs. In ena mutants, SGP circularity was unchanged in the later stages, indicating that mutant SGPs remain extended (*P<0.0001). The number of cells examined is shown in the bar. (B) SGP orientation relative to the anterior-posterior (AP) axis of the gonad was measured. At stage 13, wild-type and ena mutant SGPs were oriented along the AP axis of the gonad. At stages 15–16, the long axis of wild- type SGPs were oriented away from the AP axis; however, ena mutant SGPs remained aligned with the AP axis. The number of cells examined is shown in the middle of the graph. Error bars in (A) represent standard error.