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. 2012 Dec 26;7(12):e52672. doi: 10.1371/journal.pone.0052672

Figure 6. RT-PCR confirmation of concordant epigenes in spinal cord.

Figure 6

RNA was extracted from the postmortem human spinal cord tissue that was used for the methylation analysis from sALS (n = 8–11) subjects and controls (n = 8–11) and subjected to RT-PCR. Results were normalized to glyceraldehyde-3- phosphate dehydrogenase (GAPDH) except for STAT5A which was normalized to TATA box binding protein (Tbp) and presented as fold changes calculated by the 2−ΔΔC T method. Similar results were obtained when using different housekeeping genes (Fig. S2); *p<0.05, **p<0.01, ***p<0.001 compared to the control group (Ctrl). Data mean ± SEM is plotted using box and whiskers vertical bars plotting minimum to maximum values.