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. 2012 Dec 26;7(12):e52716. doi: 10.1371/journal.pone.0052716

Figure 1. Identification of the transcriptional start site of the USP22 gene.

Figure 1

A. Results of 5′ RACE experiments on total RNA from HeLa cells; DNA sequences were detected by gel electrophoresis using 2% agarose. Lane 1. A single DNA band of 260 bp was detected; Lane 2. DL2000 marker. B. Sequence of USP22 gene depicting the positions of the transcriptional start sites. C. Compared with the existing information in the human genome database, the DNA sequence of the HeLa clone contains two mutations, A–C and A–C, at positions 280 and 283 upstream from the transcriptional start site.